Vegetables are the major source of dietary nitrate, with wide variations in nitrate content. The presence of nitrate in vegetables is often associated with harmful effects on human health. The study investigated nitrate of vegetables in Phu My ward, Hiep An ward, Tuong Binh Hiep ward, Thu Dau Mot city, Binh Duong province in 2020 by UV-VIS for determining of nitrat concentration, measurement wavelength of 410 nm. The vegetable samples were collected in the fields according to method of TCVN 9016:2011 and the nitrate contents were determinated according to method of TCVN 8742:2011 (Vietnam Ministry of Sciences and Technology, 2011). The results showed that there were 3 fresh spinach samples, 3 bok choy samples, contained the nitrate contents exceeded the regulations according to the regulations of nitrate contents in vegetables in the decisions of world health organization site (WHO). These results warn the farmers and policy administers of Thu Dau Mot city to give the solutions of nitrate-content controling in vegetables producing in Binh Duong.