IN VITRO REGENERATION OF STRIPE EBONY (DIOSPYROS FRUTESCENS BLUME)

By Ngo Vu Nguyen, Nguyen Minh Ty
DOI: 10.37550/tdmu.EJS/2024.03.584

The Stripe ebony tree (Diospyros frutescens Blume) is considered a species in danger of extinction due to overexploitation. Therefore, there is an urgent need to regenerate and conserve the species for future generation. In this study micropropagation protocol of Diospyros frutescens Blume was developed using nature zygotic embryos. Zygotic embryos isolated from germinated on MS medium containing 0.4mg L-1 Kinetin + 1.0mg L-1 BA. There were (83.11±0.79%) of explants induced shoots after 30 days of culture. The highest number of shoot on 1/2 MS medium supplemented with 2.5mg L-1 BA + 0.6mg L-1 IBA was 5.84±0.035 shoots per explant after 45 days of culture. The percentage of shoot produced roots the highest 85.52±0.48% after 45 days culture on 1/2 MS medium contain 0.8mg L-1 IBA