Dipterocarpus dyeri is a typical plant of tropical evergreen moist forest at Southeast Vietnam. These plants have been planted popularly at parks and urban streets for the shade and it has been commonly materials for timber industry. Multiplication of Dipterocarpus dyeri at nurseries could face to some diseases, such as the withered disease cause serial death. Our study isolated three disease fungi strains from the root areas of the diseased Dipterocarpus dyeri planted Ma Da nursery, Dong Nai province. Result of 28s rDNA sequencing showed these fungi belong to Ophiostoma eucalypticagena, Aspergillus nidulans and Collectotrichum gloeosporioides. This result is base for conducting the following studies to control the withered disease on Dipterocarpus dyeri at the nursery.